281 papers
Molecular biology explores the tiny machinery inside living cells, revealing how genetic instructions are read, copied, and turned into the proteins that keep us alive. This field acts as a bridge between the static code of DNA and the dynamic processes that drive growth, disease, and life itself, offering insights into everything from cellular repair to the development of new medicines.
On Gist.Science, we track every new preprint uploaded to bioRxiv in this category to make these complex discoveries accessible to everyone. Our team processes each submission to provide both clear, plain-language explanations and detailed technical summaries, ensuring you can grasp the core findings without getting lost in dense academic jargon.
Below are the latest molecular biology papers freshly processed from bioRxiv, ready for you to explore and understand.
This study presents a cost-effective, in-house production and purification protocol for a redesigned MarathonRT enzyme that matches the performance of commercial alternatives (Induro and uMRT) in tRNA sequencing, while also introducing a rapid spin-column extraction method to further reduce the time and cost of library preparation.
This study elucidates that the circZNF827-hnRNPL/K complex transcriptionally represses NGFR and other loci enriched with H3K27me3 marks during neuronal differentiation, triggering a primary regulatory event that initiates a secondary response to further promote the differentiation process.
This speculative review synthesizes the discovery and multifunctional roles of Igf2bp proteins in nervous system development and cancer, proposing a model where these RNA-binding proteins evolved to coordinate post-transcriptional regulation in neurogenesis and were later co-opted in tumorigenesis, a hypothesis supported by evolutionary analysis revealing that genes co-evolved with Igf2bps are enriched for neural functions and overlap with oncogenic targets stabilized by Igf2bp1.
The authors developed a one-pot, isothermal MethylAmp workflow that simultaneously amplifies DNA and preserves native methylation patterns by optimizing a unified 42°C reaction condition to support both helicase-dependent amplification and DNMT1-mediated methylation, thereby enabling accurate epigenetic analysis without disrupting methylation marks.
This study demonstrates that tethering chromatin to the nuclear envelope via the LINC complex counteracts its self-attraction to prevent excessive clustering, thereby enhancing RNAPII accessibility and promoting asymmetric chromatin organization essential for efficient transcription.
A 2.5-year mesocosm experiment across eight coral species reveals that symbiont community responses to future ocean conditions exist on a continuum ranging from deterministic, thermally adaptive shifts to stochastic dysbiosis, depending on the intensity of heat stress and coral lineage.
This study introduces and validates a novel, highly sensitive, and specific qPCR assay using new primers and a probe to detect trace DNA of *Anopheles gambiae* complex mosquitoes in dietary and environmental samples, thereby enabling advances in mosquito ecological research and vector-control strategies.
This study identifies multiple cell lineages as contributors to myofibroblast-mediated subretinal fibrosis in AMD and demonstrates that restoring downregulated miR-24 or combining SMAD3 and MRTF inhibitors effectively suppresses fibrosis by targeting key TGF-β and PAK4/LIMK2 signaling pathways.
This study identifies the E3 ubiquitin ligase ITCH as a critical host factor that promotes SARS-CoV-2 replication and virion release by enhancing viral protein ubiquitination and assembly, facilitating autophagosome-dependent secretion, and suppressing protease-mediated spike protein destabilization, thereby highlighting ITCH as a promising therapeutic target.
This study reveals that ATP-competitive kinase inhibitors frequently trigger paradoxical, non-catalytic effects by inducing structural rearrangements in autoinhibitory domains that alter protein-protein interactions and subcellular localization, a phenomenon identified through a multimodal proteomics approach.